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mouse monoclonal anti rab7  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mouse monoclonal anti rab7
    Mouse Monoclonal Anti Rab7, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+rab7/anti-RAb7%2FCG5915+protein/pmc13089057-12-0-7
    Average 96 stars, based on 111 article reviews
    mouse monoclonal anti rab7 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Endocytome profiling uncovers cell-surface protein dynamics underlying neuronal connectivity
    Article Snippet: .. The following antibodies were used: rat anti-Ncad (Developmental Studies Hybridoma Bank; 1:40), chicken anti-GFP (Aves Labs; 1:1000), rabbit anti-HA (Cell Signaling Technologies; 1:200), rat anti-HA (Sigma Aldrich; 1:200), mouse anti-V5 (ThermoFisher Scientific; 1:100), guinea pig anti-Mcr ; (1:500), rabbit anti-dsRed (Takara Bio; 1:1000), rabbit anti-Lamp1 (Abcam; 1:100), rabbit anti-Rab5 (Abcam; 1:100), mouse anti-Rab7 (Developmental Studies Hybridoma Bank; 1:10), goat anti-HRP-594 (Jackson ImmunoResearch; 1:300), mouse anti-mCherry (ThermoFisher Scientific; 1:1000), mouse anti-Rab11 (BD Biosciences; 1:100), rabbit anti-Rab5 (Abcam; 1:100), and rat anti-V5 (Abcam; 1:500) and incubated with brains in block buffer overnight at 4°C while nutating. ..

    Article Title: Endocytome profiling uncovers cell-surface protein dynamics underlying neuronal connectivity
    Article Snippet: -V5, pUASt-attB-HA-HRP surf , or pUASt-attB-HA-HRP endo using Effectine (Qiagen). .. After 48 h, transfected cells were washed in 1X PBS, fixed in 4% PFA in PBS for 15 min, permeabilized in PBST, blocked in 5% normal donkey serum in PBST, and stained with: rabbit anti-HA (C29F4; Cell Signaling Technology) or rat anti-HA (3F10; Sigma Aldrich), mouse anti-Rab7 (Developmental Studies Hybridoma Bank), rabbit anti-Rab5 (Abcam); or mouse anti-V5 (ThermoFisher) or rabbit anti-V5 (ThermoFisher) at room temperature for 2 h, followed by three 15 min washes in PBST, and incubated in secondary antibody for 2 h at room temperature. ..

    Article Title: Endocytome profiling uncovers cell-surface protein dynamics underlying neuronal connectivity.
    Article Snippet: -V5, pUASt-attB-HAHRP surf , or pUASt-attB-HA-HRP endo using Effectine (Qiagen). .. After 48 h, transfected cells were washed in 1X PBS, fixed in 4% Neuron 114, 1–16.e1–e6, June 3, 2026 e5 PFA in PBS for 15 min, permeabilized in PBST, blocked in 5% normal donkey serum in PBST, and stained with: rabbit anti-HA (C29F4; Cell Signaling Technology) or rat anti-HA (3F10; Sigma Aldrich), mouse anti-Rab7 (Developmental Studies Hybridoma Bank), rabbit anti-Rab5 (Abcam); or mouse anti-V5 (ThermoFisher) or rabbit anti-V5 (ThermoFisher) at room temperature for 2 h, followed by three 15 min washes in PBST, and incubated in secondary antibody for 2 h at room temperature. ..

    Blocking Assay:

    Article Title: Endocytome profiling uncovers cell-surface protein dynamics underlying neuronal connectivity
    Article Snippet: .. The following antibodies were used: rat anti-Ncad (Developmental Studies Hybridoma Bank; 1:40), chicken anti-GFP (Aves Labs; 1:1000), rabbit anti-HA (Cell Signaling Technologies; 1:200), rat anti-HA (Sigma Aldrich; 1:200), mouse anti-V5 (ThermoFisher Scientific; 1:100), guinea pig anti-Mcr ; (1:500), rabbit anti-dsRed (Takara Bio; 1:1000), rabbit anti-Lamp1 (Abcam; 1:100), rabbit anti-Rab5 (Abcam; 1:100), mouse anti-Rab7 (Developmental Studies Hybridoma Bank; 1:10), goat anti-HRP-594 (Jackson ImmunoResearch; 1:300), mouse anti-mCherry (ThermoFisher Scientific; 1:1000), mouse anti-Rab11 (BD Biosciences; 1:100), rabbit anti-Rab5 (Abcam; 1:100), and rat anti-V5 (Abcam; 1:500) and incubated with brains in block buffer overnight at 4°C while nutating. ..

    other:

    Article Title: Endocytome profiling uncovers cell-surface protein dynamics underlying neuronal connectivity
    Article Snippet: Mouse anti-Rab7 , Developmental Studies Hybridoma Bank , RRID:AB_2722471.

    Article Title: Endocytome profiling uncovers cell-surface protein dynamics underlying neuronal connectivity.
    Article Snippet: 4 μL of each fraction was injected onto a microcapillary column (Picofrit with 10 μm tip opening/ 75 μm diameter, New Objective, PF360-75-10-N-5), packed in-house with 30 cm of C18 silica material (1.5 μm ReproSil-Pur C18-AQ medium, Dr. Maisch GmbH, r119.aq) and heated to 50 ◦ C using column heater sleeves (PhoenixST).

    Transfection:

    Article Title: Endocytome profiling uncovers cell-surface protein dynamics underlying neuronal connectivity
    Article Snippet: -V5, pUASt-attB-HA-HRP surf , or pUASt-attB-HA-HRP endo using Effectine (Qiagen). .. After 48 h, transfected cells were washed in 1X PBS, fixed in 4% PFA in PBS for 15 min, permeabilized in PBST, blocked in 5% normal donkey serum in PBST, and stained with: rabbit anti-HA (C29F4; Cell Signaling Technology) or rat anti-HA (3F10; Sigma Aldrich), mouse anti-Rab7 (Developmental Studies Hybridoma Bank), rabbit anti-Rab5 (Abcam); or mouse anti-V5 (ThermoFisher) or rabbit anti-V5 (ThermoFisher) at room temperature for 2 h, followed by three 15 min washes in PBST, and incubated in secondary antibody for 2 h at room temperature. ..

    Article Title: Endocytome profiling uncovers cell-surface protein dynamics underlying neuronal connectivity.
    Article Snippet: -V5, pUASt-attB-HAHRP surf , or pUASt-attB-HA-HRP endo using Effectine (Qiagen). .. After 48 h, transfected cells were washed in 1X PBS, fixed in 4% Neuron 114, 1–16.e1–e6, June 3, 2026 e5 PFA in PBS for 15 min, permeabilized in PBST, blocked in 5% normal donkey serum in PBST, and stained with: rabbit anti-HA (C29F4; Cell Signaling Technology) or rat anti-HA (3F10; Sigma Aldrich), mouse anti-Rab7 (Developmental Studies Hybridoma Bank), rabbit anti-Rab5 (Abcam); or mouse anti-V5 (ThermoFisher) or rabbit anti-V5 (ThermoFisher) at room temperature for 2 h, followed by three 15 min washes in PBST, and incubated in secondary antibody for 2 h at room temperature. ..

    Staining:

    Article Title: Endocytome profiling uncovers cell-surface protein dynamics underlying neuronal connectivity
    Article Snippet: -V5, pUASt-attB-HA-HRP surf , or pUASt-attB-HA-HRP endo using Effectine (Qiagen). .. After 48 h, transfected cells were washed in 1X PBS, fixed in 4% PFA in PBS for 15 min, permeabilized in PBST, blocked in 5% normal donkey serum in PBST, and stained with: rabbit anti-HA (C29F4; Cell Signaling Technology) or rat anti-HA (3F10; Sigma Aldrich), mouse anti-Rab7 (Developmental Studies Hybridoma Bank), rabbit anti-Rab5 (Abcam); or mouse anti-V5 (ThermoFisher) or rabbit anti-V5 (ThermoFisher) at room temperature for 2 h, followed by three 15 min washes in PBST, and incubated in secondary antibody for 2 h at room temperature. ..

    Article Title: Endocytome profiling uncovers cell-surface protein dynamics underlying neuronal connectivity.
    Article Snippet: -V5, pUASt-attB-HAHRP surf , or pUASt-attB-HA-HRP endo using Effectine (Qiagen). .. After 48 h, transfected cells were washed in 1X PBS, fixed in 4% Neuron 114, 1–16.e1–e6, June 3, 2026 e5 PFA in PBS for 15 min, permeabilized in PBST, blocked in 5% normal donkey serum in PBST, and stained with: rabbit anti-HA (C29F4; Cell Signaling Technology) or rat anti-HA (3F10; Sigma Aldrich), mouse anti-Rab7 (Developmental Studies Hybridoma Bank), rabbit anti-Rab5 (Abcam); or mouse anti-V5 (ThermoFisher) or rabbit anti-V5 (ThermoFisher) at room temperature for 2 h, followed by three 15 min washes in PBST, and incubated in secondary antibody for 2 h at room temperature. ..

    Dominant Negative Mutation:

    Article Title: A decline in follicle cell function is a major driver of Drosophila ovarian aging
    Article Snippet: .. Primary antibodies used were: mouse anti-Fas3 7G10 (1:100, DSHB), rat anti-Vasa 46F11 s(1:200, DSHB), rat anti-CadN DN-Ex#8 (1:100, DSHB), rabbit anti-pHH3 (Ser10, 1:500, Millipore Sigma), mouse anti-UNC93-5.2.1 (gH2Av, 1:100, DSHB), and rabbit anti-Vkg (1:1000, Dr. Stephane Noselli), rat anti-RFP 5F8 (1:500, Chromatek), mouse anti-Rab7 (1:100, DSHB). .. The following secondary antibodies were used at 1:500: goat anti-mouse 488 (Life Technologies, A11001), goat anti-rabbit 488 (Invitrogen, A11034), goat anti-rat 488 (Invitrogen, A11006), goat anti-mouse 555 (Invitrogen, A21422), goat anti-rat 555 (Invitrogen, A21434), goat anti-mouse 647 (Invitrogen, A21236), goat anti-rat 647 (Invitrogen, A21247).



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